Chicken liver transfer ribonucleic acid: heterologous interaction of alanine transfer ribonucleic acid with yeast
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Chicken liver transfer ribonucleic acid: heterologous interaction of alanine transfer ribonucleic acid with yeast.
We are attempting to determine the nature of specific RNA-protein interactions by study of tRNA charging by homologous and heterologous enzymes. tRNAklatRNARNA3 t were prepared by phenoxyacetylation of alanyl-tRNA and separation on benzoylated DEAE-cellulose (Gillam, Blew, Warrington, von Tigerstrom & Tener, 1968). A partially purified alanyl-tRNA synthetase from yeast forms alanyl-tRNAAla tand...
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The purification of the yeast alanine-, tyrosine-, and valinetransfer ribonucleic acids by countercurrent distribution has been described (l), and preliminary data have been reported on the oligonucleotide compositions of these RNAs (2, 3). The present paper summarizes results of attempts to account quantitatively for all of the fragments obtained by digestion of the alanineRNA with pancreatic ...
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Chick embryo tRNA, prepared by a simple large-scale method, was fractionated on three different ion-exchange columns. In all cases simple chromatographic patterns for various tRNA species were observed, indicating the presence of only a few major species of tRNA for each amino acid. By repeated chromatography one species of alanine tRNA was purified to approx. 80% purity. T(1) ribonuclease dige...
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Polylysyl transfer ribonucleic acid, free of lysyl-tRNA, has been isolated and its characteristics in an Escherichiu cofi polypeptide-synthesizing system in vitro have been studied. Ribosomes obtained from frozen E. coli cells were washed in 0.5 M NH&l and assayed at MgCla concentrations of at least 0.01 M. Binding of this peptidyl-tRNA to ribosomes occurs in the presence of templates containin...
متن کاملRole of isoleucyl-transfer ribonucleic acid synthetase in ribonucleic acid synthesis and enzyme repression in yeast.
Temperature-sensitive mutations in the isoleucyl-transfer ribonucleic acid (tRNA) synthetase of yeast, ilS(-)1-1 and ilS(-)1-2, were used to examine the role of aminoacyl-tRNA synthetase enzymes in the regulation of ribonucleic acid (RNA) synthesis and enzyme synthesis in a eucaryotic organism. At the permissive temperature, 70 to 100% of the intracellular isoleucyl-tRNA was charged in mutants ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1971
ISSN: 0306-3283
DOI: 10.1042/bj1210005pa